Immunodiagnosis of hepatitis B with high-affinity IgM monoclonal antibodies.
نویسندگان
چکیده
منابع مشابه
Immunodiagnosis of hepatitis B with high-affinity IgM monoclonal antibodies.
High-affinity monoclonal IgG and IgM antibodies to hepatitis B surface antigen (HBsAg) have been prepared and their functional capabilities explored by means of solid-phase radioimmunoassays. 125I-labeled HBsAg binding studies indicated that monoclonal IgM antibodies against HBsAg (anti-HBs) coupled to a solid-phase support quantitatively bound more HbsAg at a faster rate than conventionally pr...
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A monoclonal IgM antibody (18-2-3) derived from cell fusion of (NZB X NZW) F1 splenocytes following secondary immunization with fluorescein-conjugated keyhole limpet hemocyanin was shown to exhibit high intrinsic binding affinity and cryoinsolubility. Affinity-purified preparations were determined to be IgM by immunochemical, electrophoretic, and chromatographic analyses. An intrinsic associati...
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Availability of specific antihuman IgM antibodies is very important for developing diagnostic kits for infectious diseases that are highly prevalent in Indonesia. In line with such idea, an attempt to construct hybridoma cells producing antihuman IgM antibodies is done by fusing NSl myeloma cell line with immunized mouse spleen celLs. Hybridomas producing anti human IgM antibodies have been obt...
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Background: The affinity of an antibody to its antigen is a crucial parameter in its biological activity and performance of an immunoassay such as ELISA. Affinity of most IgG specific MAbs are often determined by methods which require labeling of either antigen or antibody, and are sometimes difficult to control, do not always lead to the expected signal and often result in immunological modifi...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1981
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.78.2.1214